EMBOSS explorer


Display restriction enzyme binding sites in a nucleotide sequence (read the manual)

Unshaded fields are optional and can safely be ignored. (hide optional fields)

Input section

Select an input sequence. Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:

Restriction enzyme methylation data file. Use one of the following two fields:

  1. To access a standard EMBOSS data file, enter the name here:
  2. To upload a data file from your local computer, select it here:
Required section
Comma separated enzyme list
Minimum recognition site length
Additional section
Minimum cuts per RE
Maximum cuts per RE
Force single site only cuts?
Allow blunt end cutters?
Allow sticky end cutters?
Allow ambiguous matches?
Allow circular DNA?
Use methylation data?
Only enzymes with suppliers?
Genetic code to use
Frame(s) to translate
Output section
List the enzymes that cut?
Display RE sites in flat format?
Limits reports to one isoschizomer?
Display translation?
Display cut sites and translation of reverse sense?
Minimum size of ORFs (If this value is left as 0 then all of the translation is shown.)
Regions to put in uppercase (eg: 4-57,78-94) (If this is left blank, then the sequence case is left alone.)
Regions to colour in HTML (eg: 4-57 red 78-94 green)
Display protein sequences in three-letter code?
Number the sequences?
Width of sequence to display
Line length of page (0 for indefinite)
Margin around sequence for numbering
Display sequence ID?
Display description?
Offset to start numbering the sequence from
Use HTML formatting?
Run section
Email address: If you are submitting a long job and would like to be informed by email when it finishes, enter your email address here.